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Laboratory techniques for elucidating liposome biology are described in step-by-step detail to aid researchers working in drug targeting, gene transport across plasma and nuclear membranes, and enzyme therapy for patients with genetic disorders. Each protocol is presented by an investigator familiar with its problems and capabilities, and includes a list of materials, troubleshooting tips, and pitfalls to avoid. The protocols are of interest to researchers in glycoproteins, glycolipids, antibody delivery, and protease delivery. The editors are affiliated with the Department of Chemistry and Biochemistry at the University of Notre Dame.Book News, Inc.

Book Info

Univ. of Notre Dame, Notre Dame, IN. Presents detailed laboratory protocols covering different aspects of liposome biology. Each protocol includes a notes section with troubleshooting tips. For researchers. Outline format.

Book Description

A panel of skilled researchers describes in step-by-step detail their best laboratory techniques for elucidating liposome biology. With these readily reproducible methods, investigators can illuminate such critical questions as the attachment of liposmes to cell surfaces, the permeation of liposomes through the plasma membranes, and the stability of liposomes in cellular and nuclear matrices. Each detailed protocol is presented by an investigator who is intimately familiar with its problems and capabilities, and includes a detailed list of materials to be used, troubleshooting tips straight from the benchtop, and notes on pitfalls to avoid.

Preface
Contributors
I Physical Properties
1 Preparation, Isolation, and Characterization of Liposomes Containing Natural and Synthetic Lipids 3
2 Preparation and Use of Liposomes for the Study of Sphingolipids Segregation in Membrane Model Systems 17
II Liposome Fusion/Modulation
3 Peptide-Induced Fusion of Liposomes 31
4 Liposomes: Applications in Protein - Lipid Interaction Studies 49
5 Lipids in Viral Fusion 61
III Application of Liposomes
6 Liposome-Mediated, Fluorescence-Based Studies of Sphingolipid Metabolism in Intact Cells 85
7 Micelles and Liposomes in Metabolic Enzyme and Glycolipid Glycosyltransferase Assays 107
8 Liposomes and Phospholipid Binding Proteins in Glycoprotein Biosynthesis 131
9 Therapeutic Uses of Antioxidant Liposomes 145
10 Targeted Gene Delivery by Virosomes 163
11 Liposomes Containing Ligands: Binding Specificity to Selectins 175
12 Preparation and Characterization of Glycolipid-Bearing Multilamellar and Unilamellar Liposomes 193
13 Use of Liposomes Containing Carbohydrates for Production of Monoclonal Antibodies 203
14 Inhibition of Tumor Metastasis by Liposomes Containing Glyco-Replica Peptides 219
15 Use of Phospholipid Bilayers and Monolayers in Binding Studies of Vitamin K-Dependent Blood Coagulation Proteins 233
Index 245