Abstract
Leading drosophilists describe in step-by-step detail all the essential techniques for studying Drosophila chromosomes and suggest new avenues for scientific exploration. The chapters emphasize specimen preparation (from dissection to mounting) and cover both polytene and mitotic/meiotic chromosomes in depth. Each fully tested and readily reproducible protocol offers a background introduction, equipment and reagent lists, and tips on troubleshooting and avoiding pitfalls. A cutting-edge FISH and immunolocalization technique will be important for discovering how DNA sequence influences higher-order chromosome architecture and ultimately gene expression.

From Book News, Inc.
Intended for graduate students and postdoctoral researchers, this laboratory manual explains how to prepare and study mitotic and polytene chromosomes found in the fruit fly, drosophila melanogaster, which has made significant contributions to the science of eukaryotic chromosomes. The 24 chapters provide material lists and step-by-step protocols for investigating basic questions of mitotic and meiotic cell biology and performing cytological of heterochromatin. The volume also covers Feulgen-DNA cytophotometry for estimating C values, acetic orcein staining, fluorescent BrdU labeling and nuclear flow sorting of the ovary, visualizing mitosis in whole mount larval brains, and the wing somatic mutation and recombination test.Copyright 2004 Book News, Inc., Portland, OR

Book Info
State Univ. of New York at Stony Brook. Features new, classic, and cutting-edge methods for analyzing Drosophila chromosomes and gives a comprehensive treatment of mitotic, meiotic, and polytene chromosomes. DNLM: Drosophila melanogaster--cytology--Laboratory Manuals.